cDNA expression array reveals hyperoxia-induced changes in patterns of gene expression in mouse lung
Faton H. Agani, Ismail A. Dreshaj, Musa A. Haxhiu, Richard J. Martin

The mouse model has been extensively used to characterize  patho-physiological effects of hyperoxia on the developing lung. We sought to investigate changes in gene expression in hyperoxia exposed lungs of mouse pups, and identify new candidate genes that may participate in the adaptive response.  We used a cDNA panoramic profiling technique designed to detect simultaneous changes in expression of 588 genes. In this study 12 day old C57BL/6 mice were exposed to hyperoxia for 1 and 4 days after which lung lysates underwent mRNA extraction. We identified increased expression of 39 genes and inhibition of 6 genes. Based on these results we consider the cDNA expression array technique very sensitive, reproducible and valuable approach for identification of changes in gene expression in developing respiratory structures exposed to hyperoxic stress.

Keywords: hyperoxia, gene expression, lung development

Applied Cardiopulmonary Pathophysiology 9: 374-377, 2000

Faton H. Agani, M.D.
Department of Pediatrics/Neonatology
Rainbow Babies and Children Hospital
Case Western Reserve University
Cleveland, OH 44106
USA
E-mail: fxa5@po.cwru.edu