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cryoprotectants and cardiac function Jan W. de Jong, Annewieke van den Beld & Gerard H. Zeilmaker The viability of donor hearts for implantation is limited to four hours. Cryopreservation, as used successfully in other fields, could significantly extend this time. We tested the effect of 20% dimethylsulfoxide, 20% ethylene glycol, 30% Ficoll (DEF stock solution), added to hypothermic St Thomas’ Hospital cardioplegia, on developed tension. Rat hearts (n=25) were stabilized at 5 g resting tension with a modified Tyrode solution at 37°C. Then they were perfused at 0°C for 5 min with cardioplegia, for 20 min with cardioplegia/DEF, and for 5 min with cardioplegia. Recovery of developed tension at 37°C was studied for 30 min without cardioplegia/DEF. A reciprocal relationship existed between DEF infused (10 - 40%), and function recovery (74 – 28%). At osmolarities 5x supranormal (30% DEF), function recovery was still > 50%. We conclude that exposure of hearts to high concentrations of DEF, combined with deep hypothermic, high K+-cardioplegia, is potentially useful for cryopreservation. Keywords: cardioplegia, cryoprotectant, isolated heart preparation, preservation Applied Cardiopulmonary Pathophysiology 7: 235-236, 1998 J. W. de Jong, Ph.D. |
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